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Image Search Results
Journal: bioRxiv
Article Title: Single-cell transcriptomics identifies regulation of invasive behavior in Drosophila follicle cells with polarity loss
doi: 10.1101/2022.06.09.495554
Figure Lengend Snippet: A. UMAP Plot of subdivided cluster 7 cells, superimposed with RNA velocity vectors. B. Correlation heatmap representing the Connection Specificity Index (CSI) matrix of active regulon modules. C. Heatmap of the unscaled activity scores of regulons in the subclusters of cluster 7. D. Heatmap of the differentially expressed markers in each subclusters of cluster 7 cells, scaled within +1.5 (red) and -1.5 (blue) log 2 fold change. E. Gene enrichment plot for cnc (red), Keap1 (green) and their overlap (bottom). F. Phase portraits showing dynamic behavior of genes in cluster 7 cells (colored according to subcluster identity as shown in ). Solid line represents the learned splicing dynamics while the dotted line represents the inferred steady state of gene expression. Keap1 , GstD1 and GstD2 exhibits increased transcription in cluster 7. G. Reporter expression of GstD-lacZ is detected by β-Gal expression (red) within the multilayered cells of lgl RNAi follicle cells (green; clonal boundaries are marked by the dotted white lines). Nuclei is marked by DAPI (white). Scale bars: 20 µm.
Article Snippet: The following antibodies or dyes are mentioned in this paper: DSHB: mouse anti-Arm (N27A1, 1:40 dilution), mouse anti-Cut (2B10, 1:30), rat anti-Shg (DCAD2, 1:20), mouse anti-Hnt (1G9, 1:15), rat anti-Mmp1 (1:1:1 mixture of 3B8, 3A6 and 5H7, 1:40), mouse anti-Sn (sn7C, 1:25). mouse anti-Drpr (5D14, 1:50)
Techniques: Activity Assay, Expressing
Journal: bioRxiv
Article Title: Single-cell transcriptomics identifies regulation of invasive behavior in Drosophila follicle cells with polarity loss
doi: 10.1101/2022.06.09.495554
Figure Lengend Snippet: A. Confocal Images showing GstD-lacZ expression, marked by β-Gal staining (red), in border cells of w 1118 egg chambers. Nucleus is marked by DAPI (white). Scale bars, 20 µm.
Article Snippet: The following antibodies or dyes are mentioned in this paper: DSHB: mouse anti-Arm (N27A1, 1:40 dilution), mouse anti-Cut (2B10, 1:30), rat anti-Shg (DCAD2, 1:20), mouse anti-Hnt (1G9, 1:15), rat anti-Mmp1 (1:1:1 mixture of 3B8, 3A6 and 5H7, 1:40), mouse anti-Sn (sn7C, 1:25). mouse anti-Drpr (5D14, 1:50)
Techniques: Expressing, Staining